The basic cerebrospinal fluid (CSF) parameters (cell count, CSF/ serum albumin ratio, glucose, lactate, immunoglobulin ratio, oligoclonal bands) are normal in CJD patients.
Detection of brain-derived proteins in CSF helps in the differential diagnosis. The detection of 14-3-3 proteins in CSF is of major importance.
In addition to 14-3-3 ELISA, our lab performs CSF RT-QuIC (Real-Time Quaking-Induced Conversion, RT-QuIC, see display item) for detection of pathological Prion protein. This method has recently been included in the diagnostic criteria for sporadic CJD (Lancet Neurol 2021;20:235-46). Published data indicate a sensitivity of ~90% and nearly 100% specificity (Prion 2011;5:150-3; Ann Neurol 2012;72:278-85). Our data show a sensitivity of ~89% and a specificity of >99% (Neurology 2018;91:e331-e338).
If specimens are sent in for specific diagnostic testing, the NRC requests that it be notified prior to shipping
(Cramm und Schmitz et al. Characteristic CSF Prion Seeding Efficiency in Humans with Prion Diseases. Mol Neurobiol 2014)